Ectopic expression of a bamboo SVP-like gene alters flowering time and floral organs in Arabidopsis thaliana

The Short Vegetative Phase (SVP) gene is a key regulator for floral transition and development. Although SVP-like genes have been identified characterized in many plant species, their orthologs bamboo not characterized. In this study, one SVP homolog was isolated from lei based on the P. edulis transcriptome database designated as PvSVP2. Phylogenetic analysis showed that PvSVP2 closely related to rice OsMADS47. Expression revealed widely expressed different tissues but significantly vegetative tissues. Moreover, it has higher transcript levels late stages of flower Overexpression Arabidopsis thaliana caused early flowering abnormal morphologies. Further, qRT-PCR regulating time (FT SOC1) development (AP1, AP3 PI) expressions increased transgenic A. lines correlated with expression. subcellular location both onion epidermal cells protoplast localized nucleus cytomembrane. Through yeast two-hybrid BIFC assays, we interacts PvMADS56 (a SOC1 homolog) PvVRN1 (an AP1 homolog). These results suggested may play an essential role process by PvVRN1. Besides, purified obtained recombinant protein prokaryotic inducement. Our data will provide information understand characterization function be beneficial illustration molecular mechanism flowering.